Evidence for Energy-Requiring Processes in Mast Cell Degranulation and Histamine Release in Rat Induced by Sinomenine

Mast Cell Degranulation and Histamine Release Observed in a New in Vitro System

A method is described by which mast cells harvested from the rat peritoneal cavity can be deposited on the surface of a Millipore filter without gross injury, permitting observation of morphological and chemical changes induced by a variety of agents. These isolated peritoneal mast cells respond to 48/80 and to polymyxin B but not to dextran or ovomucoid with degranulation and histamine release...

The release of histamine by sinomenine.

Sinomenine is an alkaloid contained in the root of Sinomenium acutum Rehd. et Wilson (Fam. Menishermaceae), growing wild in southern Japan, and has long been used as a home remedy for neuralgia and rheumatism. It has a chemical structure similar to that of morphine, as shown left (1). Ishiwari (2, 3), who first isolated this alkaloid and studied its pharmacological actions, found that sinomenin...

2-Aminoethoxydiphenyl borate reduces degranulation and release of cytokines in a rat mast cell line.

BACKGROUND AND OBJECTIVE 2-aminoethoxydiphenyl borate (2-APB) has been reported to be a useful pharmacological tool in the study of calcium signaling. It is used either as a membrane-permeable inhibitor for inositol 1,4,5-trisphosphate (IP3) receptors or a store-operated calcium (Ca2+) entry (SOCE) inhibitor. The present study aimed to evaluate the effects of 2-APB on degranulation and cytokine...

Potentiation by phosphatidylserine of calcium-dependent histamine release from rat mast cells induced by concanavalin A.

Mechanism of Peptide-induced Mast Cell Degranulation

Substance P and other polycationic peptides are thought to stimulate mast cell degranulation via direct activation of G proteins. We investigated the ability of extracellularly applied substance P to translocate into mast cells and the ability of intracellularly applied substance P to stimulate degranulation. In addition, we studied by reverse transcription--PCR whether substance P-specific rec...